Abstract
Selenomethionine (Sem) has been incorporated recombinantly into proteins many times to elucidate their structure and function. In this paper, we revisit incorporation via chemical protein synthesis to shed light on the mechanism of native chemical ligation. The effect of chalcogen position on ligation is investigated, and selenium-containing peptide ligation is optimized. Additionally, selective methylation is performed on selenolates in a peptide in the presence of unprotected thiols.
Original language | English |
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Pages (from-to) | 4983-4989 |
Number of pages | 7 |
Journal | Bioorganic and Medicinal Chemistry |
Volume | 25 |
Issue number | 18 |
DOIs | |
State | Published - 2017 |
Keywords
- Chemical protein synthesis
- Native chemical ligation
- Peptide methylation
- Selenocysteine
- Selenomethionine
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry