Resolving the Microalgal Gene Landscape at the Strain Level: A Novel Hybrid Transcriptome of Emiliania huxleyi CCMP3266

Martin Sperfeld, Dayana Yahalomi, Einat Segev

Research output: Contribution to journalArticlepeer-review


Microalgae are key ecological players with a complex evolutionary history. Genomic diversity, in addition to limited availability of high-quality genomes, challenge studies that aim to elucidate molecular mechanisms underlying microalgal ecophysiology. Here, we present a novel and comprehensive transcriptomic hybrid approach to generate a reference for genetic analyses and resolve the microalgal gene landscape at the strain level. The approach is demonstrated for a strain of the coccolithophore microalga Emiliania huxleyi, which is a species complex with considerable genome variability. The investigated strain is commonly studied as a model for algal-bacterial interactions and was therefore sequenced in the presence of bacteria to elicit the expression of interaction-relevant genes. We applied complementary PacBio Iso-Seq full-length cDNA and poly(A)-independent Illumina total RNA sequencing, which resulted in a de novo-assembled, near-complete hybrid transcriptome. In particular, hybrid sequencing improved the reconstruction of long transcripts and increased the recovery of full-length transcript isoforms. To use the resulting hybrid transcriptome as a reference for genetic analyses, we demonstrate a method that collapses the transcriptome into a genome-like data set, termed "synthetic genome"(sGenome). We used the sGenome as a reference to visually confirm the robustness of the CCMP3266 gene assembly, to conduct differential gene expression analysis, and to characterize novel E. huxleyi genes. The newly identified genes contribute to our understanding of E. huxleyi genome diversification and are predicted to play a role in microbial interactions. Our transcriptomic toolkit can be implemented in various microalgae to facilitate mechanistic studies on microalgal diversity and ecology.

Original languageEnglish
Article numbere01418-21
JournalApplied and Environmental Microbiology
Issue number2
Early online date10 Nov 2021
StatePublished - Jan 2022


  • Differential gene expression
  • Emiliania huxleyi (coccolithophore
  • Full-length cDNA
  • Genome variability
  • Haptophyte)
  • Hybrid transcriptome assembly
  • Microbial interactions
  • Sulfur and DMSP metabolism
  • Whole transcriptome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Ecology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Resolving the Microalgal Gene Landscape at the Strain Level: A Novel Hybrid Transcriptome of Emiliania huxleyi CCMP3266'. Together they form a unique fingerprint.

Cite this