Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin; Yaron Aviv; Hagit Aviv; Elad Goldberg; Yaakov R. Tischler

doi:https://doi.org/10.1038/s41598-017-02212-2

Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin, Yaron Aviv, Hagit Aviv, Elad Goldberg, Yaakov R. Tischler

Department of Chemistry

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enhances Gram negative bacteria.

Original language	English
Article number	3810
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-02212-2
State	Published - 1 Dec 2017

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abstract = "Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enhances Gram negative bacteria.",

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Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Abstract

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