Remote immune processes revealed by immune-derived circulating cell-free dna

Ilana Fox-Fisher; Sheina Piyanzin; Bracha Lea Ochana; Agnes Klochendler; Judith Magenheim; Ayelet Peretz; Netanel Loyfer; Joshua Moss; Daniel Cohen; Yaron Drori; Nehemya Friedman; Michal Mandelboim; Marc E. Rothenberg; Julie M. Caldwell; Mark Rochman; Arash Jamshidi; Gordon Cann; David Lavi; Tommy Kaplan; Benjamin Glaser; Ruth Shemer; Yuval Dor

doi:https://doi.org/10.7554/eLife.70520

Remote immune processes revealed by immune-derived circulating cell-free dna

Ilana Fox-Fisher, Sheina Piyanzin, Bracha Lea Ochana, Agnes Klochendler, Judith Magenheim, Ayelet Peretz, Netanel Loyfer, Joshua Moss, Daniel Cohen, Yaron Drori, Nehemya Friedman, Michal Mandelboim, Marc E. Rothenberg, Julie M. Caldwell, Mark Rochman, Arash Jamshidi, Gordon Cann, David Lavi, Tommy Kaplan, Benjamin GlaserRuth Shemer, Yuval Dor

Tel Aviv University, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.

Original language	English
Article number	e70520
Journal	eLife
Volume	10
DOIs	https://doi.org/10.7554/eLife.70520
State	Published - 29 Nov 2021

All Science Journal Classification (ASJC) codes

General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology
General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.7554/eLife.70520

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Fox-Fisher, I., Piyanzin, S., Ochana, B. L., Klochendler, A., Magenheim, J., Peretz, A., Loyfer, N., Moss, J., Cohen, D., Drori, Y., Friedman, N., Mandelboim, M., Rothenberg, M. E., Caldwell, J. M., Rochman, M., Jamshidi, A., Cann, G., Lavi, D., Kaplan, T., ... Dor, Y. (2021). Remote immune processes revealed by immune-derived circulating cell-free dna. eLife, 10, Article e70520. https://doi.org/10.7554/eLife.70520

@article{e520a0fde943434b80caaff5397eae31,

title = "Remote immune processes revealed by immune-derived circulating cell-free dna",

abstract = "Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.",

author = "Ilana Fox-Fisher and Sheina Piyanzin and Ochana, {Bracha Lea} and Agnes Klochendler and Judith Magenheim and Ayelet Peretz and Netanel Loyfer and Joshua Moss and Daniel Cohen and Yaron Drori and Nehemya Friedman and Michal Mandelboim and Rothenberg, {Marc E.} and Caldwell, {Julie M.} and Mark Rochman and Arash Jamshidi and Gordon Cann and David Lavi and Tommy Kaplan and Benjamin Glaser and Ruth Shemer and Yuval Dor",

year = "2021",

month = nov,

day = "29",

doi = "https://doi.org/10.7554/eLife.70520",

language = "الإنجليزيّة",

volume = "10",

journal = "eLife",

issn = "2050-084X",

publisher = "eLife Sciences Publications",

}

Fox-Fisher, I, Piyanzin, S, Ochana, BL, Klochendler, A , Magenheim, J, Peretz, A, Loyfer, N, Moss, J, Cohen, D, Drori, Y, Friedman, N, Mandelboim, M, Rothenberg, ME, Caldwell, JM, Rochman, M, Jamshidi, A, Cann, G, Lavi, D, Kaplan, T, Glaser, B, Shemer, R & Dor, Y 2021, 'Remote immune processes revealed by immune-derived circulating cell-free dna', eLife, vol. 10, e70520. https://doi.org/10.7554/eLife.70520

TY - JOUR

T1 - Remote immune processes revealed by immune-derived circulating cell-free dna

AU - Fox-Fisher, Ilana

AU - Piyanzin, Sheina

AU - Ochana, Bracha Lea

AU - Klochendler, Agnes

AU - Magenheim, Judith

AU - Peretz, Ayelet

AU - Loyfer, Netanel

AU - Moss, Joshua

AU - Cohen, Daniel

AU - Drori, Yaron

AU - Friedman, Nehemya

AU - Mandelboim, Michal

AU - Rothenberg, Marc E.

AU - Caldwell, Julie M.

AU - Rochman, Mark

AU - Jamshidi, Arash

AU - Cann, Gordon

AU - Lavi, David

AU - Kaplan, Tommy

AU - Glaser, Benjamin

AU - Shemer, Ruth

AU - Dor, Yuval

PY - 2021/11/29

Y1 - 2021/11/29

N2 - Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.

AB - Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.

UR - http://www.scopus.com/inward/record.url?scp=85120162200&partnerID=8YFLogxK

U2 - https://doi.org/10.7554/eLife.70520

DO - https://doi.org/10.7554/eLife.70520

M3 - مقالة

C2 - 34842142

SN - 2050-084X

VL - 10

JO - eLife

JF - eLife

M1 - e70520

ER -

Remote immune processes revealed by immune-derived circulating cell-free dna

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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