Recording Ca++ Transients in Neurons by TCSPC FLIM

Wolfgang Becker; Samuel Frere; Inna Slutsky

doi:10.1007/978-981-10-9020-2_5

Recording Ca⁺⁺ Transients in Neurons by TCSPC FLIM

Wolfgang Becker, Samuel Frere, Inna Slutsky

Physiology Pharmacology

Tel Aviv University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Multi-dimensional time-correlated single photon counting (TCSPC) techniques are able to record Ca⁺⁺ transients in live neurons via the fluorescence lifetime changes of a Ca⁺⁺-sensitive dye. The technique is based on periodical stimulation of the sample, raster scanning, or line scanning with a high-frequency pulsed laser, and multi-dimensional TCSPC. The recording process builds up a photon distribution over the spatial coordinates of the scan, the times of the photons in the laser pulse period, and the times of the photons in the stimulation period. The transient-time resolution is about 40 ms for raster scanning and about 1 ms for line scanning. We demonstrate the technique for electrical stimulation of cultured neurons incubated with Oregon Green Bapta.

Original language	English
Title of host publication	Progress in Optical Science and Photonics
Pages	103-110
Number of pages	8
DOIs	https://doi.org/10.1007/978-981-10-9020-2_5
State	Published - 2019

Publication series

Name	Progress in Optical Science and Photonics
Volume	5

Keywords

Fluorescence Lifetime Imaging (FLIM)
Lifetime Changes
Oregon Green Bapta
Pulse-periodic Laser
Time-correlated Single Photon Counting (TCSPC)

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

Access to Document

10.1007/978-981-10-9020-2_5

Cite this

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title = "Recording Ca++ Transients in Neurons by TCSPC FLIM",

abstract = "Multi-dimensional time-correlated single photon counting (TCSPC) techniques are able to record Ca++ transients in live neurons via the fluorescence lifetime changes of a Ca++-sensitive dye. The technique is based on periodical stimulation of the sample, raster scanning, or line scanning with a high-frequency pulsed laser, and multi-dimensional TCSPC. The recording process builds up a photon distribution over the spatial coordinates of the scan, the times of the photons in the laser pulse period, and the times of the photons in the stimulation period. The transient-time resolution is about 40 ms for raster scanning and about 1 ms for line scanning. We demonstrate the technique for electrical stimulation of cultured neurons incubated with Oregon Green Bapta.",

keywords = "Fluorescence Lifetime Imaging (FLIM), Lifetime Changes, Oregon Green Bapta, Pulse-periodic Laser, Time-correlated Single Photon Counting (TCSPC)",

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