@inbook{c46fe739e65c4a829d500e32e882e116,
title = "Recording and hodgkin-huxley kinetic analysis of voltage-gated ion channels in nucleated patches",
abstract = "The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.",
keywords = "Hodgkin-Huxley model, Kinetic analysis, Nucleated patch, Outside-out patch, Patch clamp, Voltage-gated channels",
author = "Mara Almog and Alon Korngreen",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = jan,
day = "1",
doi = "https://doi.org/10.1007/978-1-4939-3411-9_14",
language = "الإنجليزيّة",
series = "Neuromethods",
pages = "305--323",
booktitle = "Neuromethods",
}
