Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes

Lavi S. Bigman; Amnon Horovitz

doi:https://doi.org/10.1074/jbc.AC119.010299

Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes

Lavi S. Bigman, Amnon Horovitz

Department of Chemical and Structural Biology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

The chaperonin GroEL and its co-chaperonin GroES form both GroEL-GroES bullet-shaped and GroEL-GroES(2) football-shaped complexes. The residence time of protein substrates in the cavities of these complexes is about 10 and 1 s, respectively. There has been much controversy regarding which of these complexes is the main functional form. Here, we show using computational analysis that GroEL protein substrates have a bimodal distribution of folding times, which matches these residence times, thereby suggesting that both bullet-shaped and football-shaped complexes are functional. More generally, co-existing complexes with different stoichiometries are not mutually exclusive with respect to having a functional role and can complement each other.

Original language	English
Pages (from-to)	13527-13529
Number of pages	3
Journal	Journal of Biological Chemistry
Volume	294
Issue number	37
DOIs	https://doi.org/10.1074/jbc.AC119.010299
State	Published - 13 Sep 2019

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title = "Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes",

abstract = "The chaperonin GroEL and its co-chaperonin GroES form both GroEL-GroES bullet-shaped and GroEL-GroES(2) football-shaped complexes. The residence time of protein substrates in the cavities of these complexes is about 10 and 1 s, respectively. There has been much controversy regarding which of these complexes is the main functional form. Here, we show using computational analysis that GroEL protein substrates have a bimodal distribution of folding times, which matches these residence times, thereby suggesting that both bullet-shaped and football-shaped complexes are functional. More generally, co-existing complexes with different stoichiometries are not mutually exclusive with respect to having a functional role and can complement each other.",

author = "Bigman, {Lavi S.} and Amnon Horovitz",

note = "This work was supported by United States–Israel Binational Science Foundation Grant 2015170 and the Minerva Foundation with funding from the Federal German Ministry for Education and Research and the Kimmelman Center for Biomolecular Structure and Assembly. The authors declare that they have no conflicts of interest with the contents of this article.",

year = "2019",

month = sep,

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doi = "https://doi.org/10.1074/jbc.AC119.010299",

language = "الإنجليزيّة",

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T1 - Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes

AU - Bigman, Lavi S.

AU - Horovitz, Amnon

N1 - This work was supported by United States–Israel Binational Science Foundation Grant 2015170 and the Minerva Foundation with funding from the Federal German Ministry for Education and Research and the Kimmelman Center for Biomolecular Structure and Assembly. The authors declare that they have no conflicts of interest with the contents of this article.

PY - 2019/9/13

Y1 - 2019/9/13

N2 - The chaperonin GroEL and its co-chaperonin GroES form both GroEL-GroES bullet-shaped and GroEL-GroES(2) football-shaped complexes. The residence time of protein substrates in the cavities of these complexes is about 10 and 1 s, respectively. There has been much controversy regarding which of these complexes is the main functional form. Here, we show using computational analysis that GroEL protein substrates have a bimodal distribution of folding times, which matches these residence times, thereby suggesting that both bullet-shaped and football-shaped complexes are functional. More generally, co-existing complexes with different stoichiometries are not mutually exclusive with respect to having a functional role and can complement each other.

AB - The chaperonin GroEL and its co-chaperonin GroES form both GroEL-GroES bullet-shaped and GroEL-GroES(2) football-shaped complexes. The residence time of protein substrates in the cavities of these complexes is about 10 and 1 s, respectively. There has been much controversy regarding which of these complexes is the main functional form. Here, we show using computational analysis that GroEL protein substrates have a bimodal distribution of folding times, which matches these residence times, thereby suggesting that both bullet-shaped and football-shaped complexes are functional. More generally, co-existing complexes with different stoichiometries are not mutually exclusive with respect to having a functional role and can complement each other.

U2 - https://doi.org/10.1074/jbc.AC119.010299

DO - https://doi.org/10.1074/jbc.AC119.010299

M3 - مقالة

SN - 0021-9258

VL - 294

SP - 13527

EP - 13529

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 37

ER -

Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes

Abstract

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