Recombinant ostreolysin (rOly) inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells

Erez Israeli, Nastacia Adler Berken, Ofer Gover, Eike Waechtershaeuser, Lutz Graeve, Betty Schwartz

Research output: Contribution to journalArticlepeer-review

Abstract

Obesity is a nutrition-associated disorder result of an imbalance between energy intake and energy expenditure. Changing adipocytes differentiation patterns is considered as a strategy to treat obesity-related disorders. Recently, much interest is focused on the role of posttranslational modifications of tubulin on adipocyte differentiation. We recently demonstrated that a recombinant version of the fungal protein Ostreolysin (rOly) drastically affects metabolism of adipose tissue. The aim of the present study is to extend our understanding of the in vitro effects of rOly on different adipocytes. We demonstrate that rOly inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells. Additionally, rOly affected the gene expression levels of SQSTM1 and Collagen type 1, which are mediated by AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells. We provide a potential molecular mechanistic approach describing that the effect of rOly on adipocytes is mediated by tubulin acetylation and AMPK phosphorylation.

Original languageAmerican English
Pages (from-to)185-193
Number of pages9
JournalJournal of Functional Foods
Volume59
DOIs
StatePublished - Aug 2019

Keywords

  • AMPK
  • Adipose tissue
  • Cilia
  • Hedgehog signaling
  • Ostreolysin

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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