Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining

Roi Peretz; Hadas Mamane; Elizaveta Sterenzon; Yoram Gerchman

doi:10.1007/s10570-018-2162-z

Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining

Roi Peretz, Hadas Mamane, Elizaveta Sterenzon, Yoram Gerchman

School of Mechanical Engineering

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW. Graphical abstract: [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	971-977
Number of pages	7
Journal	Cellulose
Volume	26
Issue number	2
DOIs	https://doi.org/10.1007/s10570-018-2162-z
State	Published - 30 Jan 2019

Keywords

Calcofluor White
Cellulose nanocrystals (CNC)
Fluorescence
Whatman filter paper
quantification
quantification

All Science Journal Classification (ASJC) codes

Polymers and Plastics

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10.1007/s10570-018-2162-z

Cite this

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title = "Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining",

abstract = "Abstract: Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW. Graphical abstract: [Figure not available: see fulltext.]",

keywords = "Calcofluor White, Cellulose nanocrystals (CNC), Fluorescence, Whatman filter paper, quantification, quantification",

author = "Roi Peretz and Hadas Mamane and Elizaveta Sterenzon and Yoram Gerchman",

note = "Publisher Copyright: {\textcopyright} 2018, Springer Nature B.V.",

year = "2019",

month = jan,

day = "30",

doi = "10.1007/s10570-018-2162-z",

language = "الإنجليزيّة",

volume = "26",

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}

TY - JOUR

T1 - Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining

AU - Peretz, Roi

AU - Mamane, Hadas

AU - Sterenzon, Elizaveta

AU - Gerchman, Yoram

PY - 2019/1/30

Y1 - 2019/1/30

N2 - Abstract: Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW. Graphical abstract: [Figure not available: see fulltext.]

AB - Abstract: Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW. Graphical abstract: [Figure not available: see fulltext.]

KW - Calcofluor White

KW - Cellulose nanocrystals (CNC)

KW - Fluorescence

KW - Whatman filter paper

KW - quantification

UR - http://www.scopus.com/inward/record.url?scp=85057965827&partnerID=8YFLogxK

U2 - 10.1007/s10570-018-2162-z

DO - 10.1007/s10570-018-2162-z

M3 - مقالة

SN - 0969-0239

VL - 26

SP - 971

EP - 977

JO - Cellulose

JF - Cellulose

IS - 2

ER -

Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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