Rapid DNA hybridization reactions using isotachophoresis

M. Bercovici, C. M. Han, J. C. Liao, J. G. Santiago

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We use ITP to control and increase the rate of homogenous DNA hybridization reactions. We present a novel physical model, new validation experiments, and new demonstrations of this assay. We studied the coupled physics and chemistry of the dynamics of preconcentration, mixing, and chemical reaction kinetics under ITP. Our experimentally-validated model enables closed form solution for two-species hybridization reaction under ITP, and predicts 10,000 fold speed-up of chemical reaction rate at concentrations of order 10 pM, with greater enhancement of reaction rate at lower concentrations. We experimentally demonstrate 800 fold speed-up using ITP compared to the standard case of reaction in a simple, well mixed reaction chamber.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages717-719
Number of pages3
StatePublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • DNA hybridization
  • Isotachophoresis
  • Molecular beacons
  • Reaction kinetics

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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