@inbook{c8b04d5763f94434835677f7c296ec15,
title = "Rapid DNA-FISH in Arabidopsis thaliana Somatic Cells",
abstract = "Fluorescence in situ hybridization (FISH) technique has been widely used to detect and localize specific DNA and RNA sequences in interphase nuclei and chromosomes in animals and plants. Here, we present a protocol for localization of genomic loci in nuclei of the model plant Arabidopsis thaliana. This protocol includes several advances and adaptations to A. thaliana, including preparation of nuclei and chromosomes without the use of liquid nitrogen, and an in situ hybridization procedure that preserves chromatin structure without the use of paraformaldehyde and formamide. Simultaneous denaturation of the BAC (bacterial artificial chromosome) probe and nuclei followed by annealing at high temperature allows hybridization in less than an hour. These hybridization conditions also provide high signal to noise ratio by a small number of washes. Thus, this simplified in situ hybridization procedure is completed in one working day.",
keywords = "Arabidopsis thaliana, BAC-FISH, Chromosome preparation, Fluorescence in situ hybridization, Interphase nuclei, Molecular cytogenetics, PlantPlants",
author = "Olga Raskina and Ofir Hakim",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "https://doi.org/10.1007/978-1-0716-3766-1_17",
language = "American English",
series = "Methods in Molecular Biology",
pages = "259--270",
booktitle = "Methods in Molecular Biology",
}