Rapid antifungal susceptibility testing on silicon microwells

Christopher Heuer, Heidi Leonard, Nadav Nitzan, Ariella Lavy-Alperovitch, Naama Massad-Ivanir, Janina Bahnemann, Thomas Scheper, Ester Segal

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, we present a sensing system for rapid antifungal susceptibility testing (AFST). The sensor is based on silicon-based diffraction gratings; their microstructure allows to capture the fungal conidia and monitor their subsequent growth on a solid-liquid interface. As a proof of concept, we use Aspergillus Niger (A. Niger) and study its growth and response to common antifungals (at varying concentrations) by monitoring changes in the intensity of reflected light from the grating. The assay is employed for the determination of minimum inhibitory concentration (MIC) values and allows expeditious AFST within 12 h, compared to ~1-2 days using gold standard methods.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages601-602
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4 Oct 20209 Oct 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period4/10/209/10/20

Keywords

  • Antifungal Susceptibility Testing
  • Sensors
  • Silicon Microwell Diffraction Gratings

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Control and Systems Engineering

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