TY - JOUR
T1 - Quantitative surface plasmon spectroscopy
T2 - Determination of the infrared optical constants of living cells
AU - Zilbershtein, Alexander
AU - Golosovsky, Michael
AU - Lirtsman, Vladislav
AU - Aroeti, Benjamin
AU - Davidov, Dan
N1 - Funding Information: We are grateful to V. Freilikher for discussions of surface plasmon scattering in cell layers. This research was supported by Israeli Ministry of Industry and Trade through the Nofar program. V. Lirtsman gratefully acknowledges financial support from the Lady Davis Fellowship foundation .
PY - 2012/7
Y1 - 2012/7
N2 - Fourier transform infrared (FTIR) spectroscopy gives a great amount of information on the distribution of chemical components in biological objects, in particular cells, in a label-free manner. We report on the development of the spectroscopic technique that combines the surface plasmon resonance (SPR) and the FTIR. Our method is based on the original processing of SPR measurements at varying angles and wavelengths and yields the complex refractive index of the analyte in a broad wavelength region. Contrary to previous SPR studies which yielded information about refractive index variation only, our technique gives absolute optical constants of the examined medium. Using this approach, we studied living Madin Darby canine kidney (MDCK) epithelial cells cultured in their natural aqueous environment and measured their optical constants. We showed that our technique has the ability to distinguish absorption lines of certain chemical components of the cells, such as the absorption lines of a CH n bonds which are characteristic mostly for cell membrane lipids.
AB - Fourier transform infrared (FTIR) spectroscopy gives a great amount of information on the distribution of chemical components in biological objects, in particular cells, in a label-free manner. We report on the development of the spectroscopic technique that combines the surface plasmon resonance (SPR) and the FTIR. Our method is based on the original processing of SPR measurements at varying angles and wavelengths and yields the complex refractive index of the analyte in a broad wavelength region. Contrary to previous SPR studies which yielded information about refractive index variation only, our technique gives absolute optical constants of the examined medium. Using this approach, we studied living Madin Darby canine kidney (MDCK) epithelial cells cultured in their natural aqueous environment and measured their optical constants. We showed that our technique has the ability to distinguish absorption lines of certain chemical components of the cells, such as the absorption lines of a CH n bonds which are characteristic mostly for cell membrane lipids.
KW - FT-IR spectroscopy
KW - Living cells spectroscopy
KW - Optical constants
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=84861096642&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.vibspec.2012.01.019
DO - https://doi.org/10.1016/j.vibspec.2012.01.019
M3 - مقالة
SN - 0924-2031
VL - 61
SP - 43
EP - 49
JO - Vibrational Spectroscopy
JF - Vibrational Spectroscopy
ER -