@inbook{d7ed1a0e63d5435a8236cebba7397460,
title = "Quantitative measurement of translesion DNA synthesis in mammalian cells",
abstract = "Translesion DNA synthesis (TLS) is a DNA damage tolerance mechanism, in which specialized low-fidelity DNA polymerases bypass lesions that interfere with replication. This process is inherently mutagenic due to the miscoding nature of DNA lesions, but it prevents double strand breaks, genome instability, and cancer. We describe here a quantitative method for measuring TLS in mammalian cells, based on non-replicating plasmids that carry a defined and site-specific DNA lesion in a single-stranded DNA region opposite a gap. The assay is responsive to the cellular composition of TLS DNA polymerases, and TLS regulators. It can be used with a broad variety of cultured mammalian cells, and is amenable to RNAi gene silencing, making it a useful tool in the study of TLS in mammalian cells.",
keywords = "Carcinogenesis, DNA damage tolerance, DNA repair, Gapped plasmid, Mutagenesis, Site-specific DNA damage, TLS, Translesion DNA Synthesis",
author = "Omer Ziv and Noam Diamant and Sigal Shachar and Ayal Hendel and Zvi Livneh",
note = "ZL is the incumbent of the Maxwell Ellis Professorial Chair in Biomedical Research. This work was supported by grants to ZL from the Flight Attendant Medical Research Institute, Florida, USA, the Leona M. and Harry B. Helmsley Charitable Trust, NY, USA, and the Israel Science Foundation (no. 564/04 and 1136/08).",
year = "2012",
month = jul,
day = "23",
doi = "10.1007/978-1-61779-998-3_35",
language = "الإنجليزيّة",
isbn = "9781617799976",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "529--542",
editor = "Lotte Bjergb{\ae}k",
booktitle = "DNA Repair Protocols",
address = "الولايات المتّحدة",
}
