Quantitative identification of senescent cells in cancer

Anat Biran, Ziv Porat, Valery Krizhanovsky

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Cellular senescence, a state of permanent growth arrest, is an important mechanism preventing the propagation of damaged cells. It suppresses cancer development in premalignant lesions in response to activated oncogenes and in tumors following therapy. The presence of senescent cells in premalignant lesions and tumors is controlled by the immune system. The ability to identify and quantify senescent cells more efficiently in vivo is necessary in order to evaluate the effect of these cells on tumorigenesis and cancer therapy. Through combining senescent-associated beta-galactosidase staining with ImageStream X analysis, we have developed an effective method to identify and quantify senescent cancer cells in vivo.

Original languageEnglish
Title of host publicationCancer Immunosurveillance
Subtitle of host publicationMethods and Protocols
EditorsAlejandro López-Soto, Alicia R. Folgueras
PublisherHumana Press
Chapter18
Pages259-267
Number of pages9
DOIs
StatePublished - 22 Nov 2018

Publication series

NameMethods in Molecular Biology
Volume1884
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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