@inbook{c96c1efcc6bd4c979e9c560506764e4c,
title = "Quantification of dicer activity in mammalian cell lysates using a non-radioactive fluorescence method",
abstract = "In this chapter, we provide a protocol for design and usage of an in vitro, cell-free Dicing assay. Our assay is based on previously established methods and improves the ability to quantitatively measure the catalytic efficacy of the Dicer complex under various cellular conditions via non-radioactive, emitted fluorescence signal readout. Because radioactive labeling is substituted by measuring fluorescent signal the method is easy to handle. Additionally, the use of a defined amount of Cy3-quenched double-stranded substrate allows for precise detection of small changes in cellular Dicing complex activity. This experimental approach might be valuable for investigating cellular miRNA biogenesis and activity under various conditions in health and disease.",
author = "Anna Emde and Natalia Rivkin and Behlke, {Mark A.} and Eran Hornstein",
note = "Publisher Copyright: {\textcopyright} 2016, Springer Science+Business Media New York.",
year = "2016",
month = nov,
day = "15",
doi = "10.1007/7657_2016_7",
language = "الإنجليزيّة",
series = "Neuromethods",
publisher = "Humana Press",
pages = "21--27",
editor = "{Jeong Kye}, Min",
booktitle = "MicroRNA Technologies",
address = "الولايات المتّحدة",
}