Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit

Aleksey Firsov, Lyubov Shaloiko, Oleg Kozlov, Leonid Vinokurov, Alexander Vainstein, Sergey Dolgov

Research output: Contribution to journalArticlepeer-review

Abstract

Thaumatin, a supersweet protein from the African plant katemfe (Thaumatococcus daniellii Benth.), is a promising zero-calorie sweetener for use in the food and pharmaceutical industries. Due to limited natural sources of thaumatin, its production using transgenic plants is an advantageous alternative. We report a simple protocol for purification of recombinant thaumatin II from transgenic tomato. Thaumatin was extracted from ripe tomato fruit in a low-salt buffer and purified on an SP-Sephacryl column. Recombinant thaumatin yield averaged 50 mg/kg fresh fruit. MALDI-MS analysis showed correct processing of thaumatin in tomato plants. The recombinant thaumatin was indistinguishable from the native protein in a taste test. The purified tomato-derived thaumatin had an intrinsic sweetness with a threshold value in taste tests of around 50 nM. These results demonstrate the potential of an expression system based on transgenic tomato plants for production of recombinant thaumatin for the food and pharmaceutical industries.

Original languageAmerican English
Pages (from-to)1-5
Number of pages5
JournalProtein Expression and Purification
Volume123
DOIs
StatePublished - 1 Jul 2016

Keywords

  • Recombinant thaumatin II purification
  • Supersweet proteins
  • Thaumatin II
  • Transgenic tomato

All Science Journal Classification (ASJC) codes

  • Biotechnology

Fingerprint

Dive into the research topics of 'Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit'. Together they form a unique fingerprint.

Cite this