Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1

Sharon Schlesinger; Andreia H. Lee; Gary Z. Wang; Lisa Green; Stephen P. Goff

doi:10.1016/j.celrep.2013.06.003

Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1

Sharon Schlesinger, Andreia H. Lee, Gary Z. Wang, Lisa Green, Stephen P. Goff

Research output: Contribution to journal › Article › peer-review

Abstract

Embryonic cells transcriptionally repress the expression of endogenous and exogenous retroelements. Trim28, a key player in this silencing, is known to act in a large DNA-bound complex, but the other components of the complex are not fully characterized. Here, we show that the zinc finger protein Yin Yang 1 (YY1) is one such component. YY1 binds to the long terminal repeat (LTR) region of both exogenous and endogenous retroviruses (ERVs). Deletion of the YY1-binding site from the retroviral genome leads to a major loss of silencing in embryonic cells and a coordinated loss of repressive histone marks from the proviral chromatin. Depletion of YY1 protein results in marked upregulation of expression of exogenous viruses and of selected ERVs. Finally, we report an embryonic cell-specific interaction between YY1 and Trim28. Our results suggest a major role for YY1 in the silencing of both exogenous retroviruses and ERVs in embryonic cells.

Original language	English
Pages (from-to)	50-58
Number of pages	9
Journal	Cell Reports
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2013.06.003
State	Published - 11 Jul 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.celrep.2013.06.003

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@article{047c8292b2cf4bb4aa3b9b0da04b08ba,

title = "Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1",

abstract = "Embryonic cells transcriptionally repress the expression of endogenous and exogenous retroelements. Trim28, a key player in this silencing, is known to act in a large DNA-bound complex, but the other components of the complex are not fully characterized. Here, we show that the zinc finger protein Yin Yang 1 (YY1) is one such component. YY1 binds to the long terminal repeat (LTR) region of both exogenous and endogenous retroviruses (ERVs). Deletion of the YY1-binding site from the retroviral genome leads to a major loss of silencing in embryonic cells and a coordinated loss of repressive histone marks from the proviral chromatin. Depletion of YY1 protein results in marked upregulation of expression of exogenous viruses and of selected ERVs. Finally, we report an embryonic cell-specific interaction between YY1 and Trim28. Our results suggest a major role for YY1 in the silencing of both exogenous retroviruses and ERVs in embryonic cells.",

author = "Sharon Schlesinger and Lee, {Andreia H.} and Wang, {Gary Z.} and Lisa Green and Goff, {Stephen P.}",

note = "Funding Information: This work was supported by NCI grant R37 CA 30488 and NYSTEM grant N08G-152/contract #C024329 from the New York State Department of Health. S.P.G. is a Howard Hughes Medical Institute Investigator. We thank for Prisma Lopez and Stella Chung for experimental assistance.",

year = "2013",

month = jul,

day = "11",

doi = "10.1016/j.celrep.2013.06.003",

language = "الإنجليزيّة",

volume = "4",

pages = "50--58",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1

AU - Schlesinger, Sharon

AU - Lee, Andreia H.

AU - Wang, Gary Z.

AU - Green, Lisa

AU - Goff, Stephen P.

N1 - Funding Information: This work was supported by NCI grant R37 CA 30488 and NYSTEM grant N08G-152/contract #C024329 from the New York State Department of Health. S.P.G. is a Howard Hughes Medical Institute Investigator. We thank for Prisma Lopez and Stella Chung for experimental assistance.

PY - 2013/7/11

Y1 - 2013/7/11

N2 - Embryonic cells transcriptionally repress the expression of endogenous and exogenous retroelements. Trim28, a key player in this silencing, is known to act in a large DNA-bound complex, but the other components of the complex are not fully characterized. Here, we show that the zinc finger protein Yin Yang 1 (YY1) is one such component. YY1 binds to the long terminal repeat (LTR) region of both exogenous and endogenous retroviruses (ERVs). Deletion of the YY1-binding site from the retroviral genome leads to a major loss of silencing in embryonic cells and a coordinated loss of repressive histone marks from the proviral chromatin. Depletion of YY1 protein results in marked upregulation of expression of exogenous viruses and of selected ERVs. Finally, we report an embryonic cell-specific interaction between YY1 and Trim28. Our results suggest a major role for YY1 in the silencing of both exogenous retroviruses and ERVs in embryonic cells.

AB - Embryonic cells transcriptionally repress the expression of endogenous and exogenous retroelements. Trim28, a key player in this silencing, is known to act in a large DNA-bound complex, but the other components of the complex are not fully characterized. Here, we show that the zinc finger protein Yin Yang 1 (YY1) is one such component. YY1 binds to the long terminal repeat (LTR) region of both exogenous and endogenous retroviruses (ERVs). Deletion of the YY1-binding site from the retroviral genome leads to a major loss of silencing in embryonic cells and a coordinated loss of repressive histone marks from the proviral chromatin. Depletion of YY1 protein results in marked upregulation of expression of exogenous viruses and of selected ERVs. Finally, we report an embryonic cell-specific interaction between YY1 and Trim28. Our results suggest a major role for YY1 in the silencing of both exogenous retroviruses and ERVs in embryonic cells.

UR - http://www.scopus.com/inward/record.url?scp=84892437617&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2013.06.003

DO - 10.1016/j.celrep.2013.06.003

M3 - مقالة

C2 - 23810560

SN - 2211-1247

VL - 4

SP - 50

EP - 58

JO - Cell Reports

JF - Cell Reports

IS - 1

ER -

Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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