@article{4734a1054a1e4b16acaf0f27b674b11d,
title = "Protocol for studying microbiome impact on host energy and reproduction in Drosophila",
abstract = "Drosophila gut microbiome in flies has been shown to have a systemic influence on energy production by the host and the energetic investment in growth and reproduction. Here we describe a protocol for studying the mechanisms responsible for this remote regulation by gut bacteria. This protocol enables whole-body and ovary-specific quantification of energy-storing molecules as well as identification of host metabolites and pathways that are regulated by gut microbiome-derived factors. Similar procedures are applicable to additional treatments and genetic manipulations. For complete details on the use and execution of this protocol, please refer to Gnainsky et al. (2021).",
author = "Yulia Gnainsky and Maxim Itkin and Tevie Mehlman and Alexander Brandis and Sergey Malitsky and Yoav Soen",
note = "This work was supported by the Sir John Templeton Foundation (grants no. 40663 and 61122) and by a research grant from Madame Olga Klein ? Astrachan. Metabolomics profiling was supported by Vera and John Schwartz Family Center for Metabolic Biology. We thank the Weizmann Fly's core facility, metabolic profiling unit, and targeted metabolomics unit of Life Sciences Core Facilities for providing technical support. Y.G. and Y.S. designed and supervised the study. Y.G. conducted experiments and data analysis. M.I. and S.M. constructed and implemented metabolomics and lipidomics analysis. A.B. and T.M. performed measurements of ATP and ADP. Y.G. M.I. S.M. A.B. and Y.S. wrote the manuscript. The authors declare no competing interests.",
year = "2022",
month = jun,
day = "17",
doi = "https://doi.org/10.1016/j.xpro.2022.101253",
language = "الإنجليزيّة",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}