Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Hadil Onallah; Ortal Yerushalmy; Ron Braunstein; Sivan Alkalay-Oren; Amit Rimon; Daniel Gelman; Shunit Coppenhagen-Glazer; Ronen Hazan; Ran Nir-Paz

doi:10.1016/j.xpro.2024.102949

Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Hadil Onallah, Ortal Yerushalmy, Ron Braunstein, Sivan Alkalay-Oren, Amit Rimon, Daniel Gelman, Shunit Coppenhagen-Glazer, Ronen Hazan, Ran Nir-Paz

Institute of Biomedical Research at the Faculty of Dental Medicine

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.¹^,²

Original language	English
Article number	102949
Journal	STAR Protocols
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2024.102949
State	Published - 21 Jun 2024

Keywords

Clinical Protocol
Health Sciences
Microbiology

All Science Journal Classification (ASJC) codes

General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology
General Neuroscience

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10.1016/j.xpro.2024.102949

Cite this

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abstract = "Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.1,2",

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AU - Onallah, Hadil

AU - Yerushalmy, Ortal

AU - Braunstein, Ron

AU - Alkalay-Oren, Sivan

AU - Rimon, Amit

AU - Gelman, Daniel

AU - Coppenhagen-Glazer, Shunit

AU - Hazan, Ronen

AU - Nir-Paz, Ran

PY - 2024/6/21

Y1 - 2024/6/21

N2 - Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.1,2

AB - Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.1,2

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KW - Microbiology

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DO - 10.1016/j.xpro.2024.102949

M3 - مقالة

C2 - 38691464

SN - 2666-1667

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Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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