Protocol for parotidectomy and saliva analysis in mice

Khaled Zubeidat; Yasmin Saba; Or Barel; Fady L. Shoukair; Avi Hai Hovav

doi:10.1016/j.xpro.2021.101048

Protocol for parotidectomy and saliva analysis in mice

Khaled Zubeidat, Yasmin Saba, Or Barel, Fady L. Shoukair, Avi Hai Hovav

Institute of Biomedical Research at the Faculty of Dental Medicine

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

Original language	English
Article number	101048
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.101048
State	Published - 18 Mar 2022

Keywords

Immunology
Microbiology
Model Organisms

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2021.101048

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title = "Protocol for parotidectomy and saliva analysis in mice",

abstract = "The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).",

keywords = "Immunology, Microbiology, Model Organisms",

author = "Khaled Zubeidat and Yasmin Saba and Or Barel and Shoukair, {Fady L.} and Hovav, {Avi Hai}",

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AU - Saba, Yasmin

AU - Barel, Or

AU - Shoukair, Fady L.

AU - Hovav, Avi Hai

PY - 2022/3/18

Y1 - 2022/3/18

N2 - The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

AB - The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

KW - Immunology

KW - Microbiology

KW - Model Organisms

UR - http://www.scopus.com/inward/record.url?scp=85121808972&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2021.101048

DO - 10.1016/j.xpro.2021.101048

M3 - مقالة

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SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101048

ER -

Protocol for parotidectomy and saliva analysis in mice

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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