Protocol for high-content screening for the impact of overexpressed MicroRNAs on primary motor neurons

Tal Yardeni, Eran Hornstein

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

In this chapter we provide a protocol for the design and usage of automated, high-content microscopy screening that enables the investigation of microRNA (miRNA) impact on primary motor neuron. High-content screening (HCS) platforms facilitate superior precision in research and are scalable to study in parallel multiple genetic, molecular, or cellular conditions. miRNAs are critical for neuronal function and for brain integrity and are considered attractive candidate targets for therapy in many neuropathologies. Therefore, HCS platforms provide a novel paradigm for exploring the impact of miRNA expression, applicable for functional pathways discovery in an academic setting, or towards development of therapeutics in the pharma industry.

Original languageEnglish
Title of host publicationMicroRNA Technologies
EditorsMin Jeong Kye
PublisherHumana Press
Chapter1
Pages11-19
Number of pages9
DOIs
StatePublished - 15 Nov 2016

Publication series

NameNeuromethods
Volume128
ISSN (Print)0893-2336

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • General Pharmacology, Toxicology and Pharmaceutics
  • Psychiatry and Mental health

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