Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model

Shani Talice; Shany K. Barkan; Grace A. Snyder; Itamar Kozlovski; Ton Sharoni; Tom Levy; Magda Lewandowska; David Ehrens; Eliya Sultan; Alona Azarov; Shelly Oisher; Ronnie Yannai; Reuven Aharoni; Uzi Hadad; Claytus Davis; Yehu Moran; Orly Gershoni-Yahalom; Nikki Traylor-Knowles; Benyamin Rosental

doi:10.1016/j.xpro.2025.103913

Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model

Shani Talice, Shany K. Barkan, Grace A. Snyder, Itamar Kozlovski, Ton Sharoni, Tom Levy, Magda Lewandowska, David Ehrens, Eliya Sultan, Alona Azarov, Shelly Oisher, Ronnie Yannai, Reuven Aharoni, Uzi Hadad, Claytus Davis, Yehu Moran, Orly Gershoni-Yahalom, Nikki Traylor-Knowles, Benyamin Rosental

Ben-Gurion University of the Negev, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.¹

Original language	American English
Article number	103913
Journal	STAR Protocols
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2025.103913
State	Published - 19 Sep 2025

Keywords

Cell isolation
Cell-based Assays
Evolutionary biology
Flow Cytometry
Immunology
Model Organisms

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2025.103913

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Talice, S., Barkan, S. K., Snyder, G. A., Kozlovski, I., Sharoni, T., Levy, T., Lewandowska, M., Ehrens, D., Sultan, E., Azarov, A., Oisher, S., Yannai, R., Aharoni, R., Hadad, U., Davis, C., Moran, Y., Gershoni-Yahalom, O., Traylor-Knowles, N., & Rosental, B. (2025). Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. STAR Protocols, 6(3), Article 103913. https://doi.org/10.1016/j.xpro.2025.103913

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title = "Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model",

abstract = "Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.1",

keywords = "Cell isolation, Cell-based Assays, Evolutionary biology, Flow Cytometry, Immunology, Model Organisms",

author = "Shani Talice and Barkan, \{Shany K.\} and Snyder, \{Grace A.\} and Itamar Kozlovski and Ton Sharoni and Tom Levy and Magda Lewandowska and David Ehrens and Eliya Sultan and Alona Azarov and Shelly Oisher and Ronnie Yannai and Reuven Aharoni and Uzi Hadad and Claytus Davis and Yehu Moran and Orly Gershoni-Yahalom and Nikki Traylor-Knowles and Benyamin Rosental",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = sep,

day = "19",

doi = "10.1016/j.xpro.2025.103913",

language = "American English",

volume = "6",

journal = "STAR Protocols",

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Talice, S, Barkan, SK, Snyder, GA, Kozlovski, I, Sharoni, T, Levy, T, Lewandowska, M, Ehrens, D, Sultan, E, Azarov, A, Oisher, S, Yannai, R, Aharoni, R, Hadad, U, Davis, C , Moran, Y, Gershoni-Yahalom, O, Traylor-Knowles, N & Rosental, B 2025, 'Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model', STAR Protocols, vol. 6, no. 3, 103913. https://doi.org/10.1016/j.xpro.2025.103913

TY - JOUR

T1 - Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model

AU - Talice, Shani

AU - Barkan, Shany K.

AU - Snyder, Grace A.

AU - Kozlovski, Itamar

AU - Sharoni, Ton

AU - Levy, Tom

AU - Lewandowska, Magda

AU - Ehrens, David

AU - Sultan, Eliya

AU - Azarov, Alona

AU - Oisher, Shelly

AU - Yannai, Ronnie

AU - Aharoni, Reuven

AU - Hadad, Uzi

AU - Davis, Claytus

AU - Moran, Yehu

AU - Gershoni-Yahalom, Orly

AU - Traylor-Knowles, Nikki

AU - Rosental, Benyamin

PY - 2025/9/19

Y1 - 2025/9/19

N2 - Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.1

AB - Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.1

KW - Cell isolation

KW - Cell-based Assays

KW - Evolutionary biology

KW - Flow Cytometry

KW - Immunology

KW - Model Organisms

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U2 - 10.1016/j.xpro.2025.103913

DO - 10.1016/j.xpro.2025.103913

M3 - Article

C2 - 40543036

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 103913

ER -

Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model

Abstract

Keywords

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