Abstract
This paper introduces a compact, portable, and highly accurate triggering control system for a 3D confocal spinning-disk image scanning microscope (CSD-ISM). Building upon on our previously published research, we expanded the hardware of the controller and synchronized it with a sub-micron translator which scans the object in the z-direction. As well as expanding the hardware, the software also was extended from previously published work similarly as it is stated for hardware while allowing full control over the 3D movement. We showed a clear and smooth 3D image made up of a collection of 2D images at different heights.
Original language | English |
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Article number | 113888 |
Journal | Ultramicroscopy |
Volume | 257 |
DOIs | |
State | Published - Mar 2024 |
Keywords
- Fluorescence microscopy
- Image scanning microscope
- Spinning-disk microscope
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Instrumentation