Probing the interactions of intrinsically disordered proteins using nanoparticle tags

Stive Pregent; Amir Lichtenstein; Ram Avinery; Adi Laser-Azogui; Fernando Patolsky; Roy Beck

doi:10.1021/acs.nanolett.5b00073

Probing the interactions of intrinsically disordered proteins using nanoparticle tags

Stive Pregent, Amir Lichtenstein, Ram Avinery, Adi Laser-Azogui, Fernando Patolsky, Roy Beck

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

The structural plasticity of intrinsically disordered proteins serves as a rich area for scientific inquiry. Such proteins lack a fix three-dimensional structure but can interact with multiple partners through numerous weak bonds. Nevertheless, this intrinsic plasticity possesses a challenging hurdle in their characterization. We underpin the intermolecular interactions between intrinsically disordered neurofilaments in various hydrated conditions, using grafted gold nanoparticle (NP) tags. Beyond its biological significance, this approach can be applied to modify the surface interaction of NPs for the creation of future tunable "smart" hybrid biomaterials.

Original language	English
Pages (from-to)	3080-3087
Number of pages	8
Journal	Nano Letters
Volume	15
Issue number	5
DOIs	https://doi.org/10.1021/acs.nanolett.5b00073
State	Published - 13 May 2015

Keywords

Gold nanoparticles
intrinsically disordered proteins
neurofilaments
small-angle X-ray scattering
spectroscopy

All Science Journal Classification (ASJC) codes

General Chemistry
Condensed Matter Physics
Mechanical Engineering
Bioengineering
General Materials Science

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10.1021/acs.nanolett.5b00073

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title = "Probing the interactions of intrinsically disordered proteins using nanoparticle tags",

abstract = "The structural plasticity of intrinsically disordered proteins serves as a rich area for scientific inquiry. Such proteins lack a fix three-dimensional structure but can interact with multiple partners through numerous weak bonds. Nevertheless, this intrinsic plasticity possesses a challenging hurdle in their characterization. We underpin the intermolecular interactions between intrinsically disordered neurofilaments in various hydrated conditions, using grafted gold nanoparticle (NP) tags. Beyond its biological significance, this approach can be applied to modify the surface interaction of NPs for the creation of future tunable {"}smart{"} hybrid biomaterials.",

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AU - Pregent, Stive

AU - Lichtenstein, Amir

AU - Avinery, Ram

AU - Laser-Azogui, Adi

AU - Patolsky, Fernando

AU - Beck, Roy

PY - 2015/5/13

Y1 - 2015/5/13

N2 - The structural plasticity of intrinsically disordered proteins serves as a rich area for scientific inquiry. Such proteins lack a fix three-dimensional structure but can interact with multiple partners through numerous weak bonds. Nevertheless, this intrinsic plasticity possesses a challenging hurdle in their characterization. We underpin the intermolecular interactions between intrinsically disordered neurofilaments in various hydrated conditions, using grafted gold nanoparticle (NP) tags. Beyond its biological significance, this approach can be applied to modify the surface interaction of NPs for the creation of future tunable "smart" hybrid biomaterials.

AB - The structural plasticity of intrinsically disordered proteins serves as a rich area for scientific inquiry. Such proteins lack a fix three-dimensional structure but can interact with multiple partners through numerous weak bonds. Nevertheless, this intrinsic plasticity possesses a challenging hurdle in their characterization. We underpin the intermolecular interactions between intrinsically disordered neurofilaments in various hydrated conditions, using grafted gold nanoparticle (NP) tags. Beyond its biological significance, this approach can be applied to modify the surface interaction of NPs for the creation of future tunable "smart" hybrid biomaterials.

KW - Gold nanoparticles

KW - intrinsically disordered proteins

KW - neurofilaments

KW - small-angle X-ray scattering

KW - spectroscopy

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DO - 10.1021/acs.nanolett.5b00073

M3 - مقالة

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VL - 15

SP - 3080

EP - 3087

JO - Nano Letters

JF - Nano Letters

IS - 5

ER -

Probing the interactions of intrinsically disordered proteins using nanoparticle tags

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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