Preparation of BAC libraries from marine microbial populations

Gazalah Sabehi, Oded Beja

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

A protocol is presented here for the construction of BAC (bacterial artificial chromosome) libraries from planktonic microbial communities collected in marine environments. The protocol describes the collection and preparation of the planktonic microbial cells, high molecular weight DNA purification from those cells, the preparation of the BAC vector, and the special ligation and electrotransformation procedures required for successful library preparation. With small modifications, this protocol can be applied to microbes collected from other environments.

Original languageEnglish
Title of host publicationMicrobial Metagenomics, Metatranscriptomics, and Metaproteomics
Pages111-122
Number of pages12
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume531

Keywords

  • BAC
  • BAC libraries
  • DNA
  • High molecular weight
  • Pulse field gel electrophoresis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry

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