Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

Yoav Shechtman; Lucien E. Weiss; Adam S. Backer; Steffen J. Sahl; W. E. Moerner

doi:10.1021/acs.nanolett.5b01396

Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

Yoav Shechtman, Lucien E. Weiss, Adam S. Backer, Steffen J. Sahl, W. E. Moerner

Research output: Contribution to journal › Article › peer-review

Abstract

We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. (Figure Presented).

Original language	English
Pages (from-to)	4194-4199
Number of pages	6
Journal	Nano Letters
Volume	15
Issue number	6
DOIs	https://doi.org/10.1021/acs.nanolett.5b01396
State	Published - 10 Jun 2015
Externally published	Yes

Keywords

3D imaging
Nanoscopy
PSF engineering
Single particle tracking
Single-molecule imaging
Super-resolution microscopy

All Science Journal Classification (ASJC) codes

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.5b01396

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title = "Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions",

abstract = "We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. (Figure Presented).",

keywords = "3D imaging, Nanoscopy, PSF engineering, Single particle tracking, Single-molecule imaging, Super-resolution microscopy",

author = "Yoav Shechtman and Weiss, {Lucien E.} and Backer, {Adam S.} and Sahl, {Steffen J.} and Moerner, {W. E.}",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

month = jun,

day = "10",

doi = "10.1021/acs.nanolett.5b01396",

language = "الإنجليزيّة",

volume = "15",

pages = "4194--4199",

journal = "Nano Letters",

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publisher = "American Chemical Society",

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T1 - Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

AU - Shechtman, Yoav

AU - Weiss, Lucien E.

AU - Backer, Adam S.

AU - Sahl, Steffen J.

AU - Moerner, W. E.

PY - 2015/6/10

Y1 - 2015/6/10

N2 - We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. (Figure Presented).

AB - We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. (Figure Presented).

KW - 3D imaging

KW - Nanoscopy

KW - PSF engineering

KW - Single particle tracking

KW - Single-molecule imaging

KW - Super-resolution microscopy

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DO - 10.1021/acs.nanolett.5b01396

M3 - مقالة

SN - 1530-6984

VL - 15

SP - 4194

EP - 4199

JO - Nano Letters

JF - Nano Letters

IS - 6

ER -

Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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