Post-Maturation Reinforcement of 3D-Printed Vascularized Cardiac Tissues

Eric Silberman, Hadas Oved, Michael Namestnikov, Assaf Shapira, Tal Dvir

Research output: Contribution to journalArticlepeer-review


Despite advances in biomaterials engineering, a large gap remains between the weak mechanical properties that can be achieved with natural materials and the strength of synthetic materials. Here, a method is presented for reinforcing an engineered cardiac tissue fabricated from differentiated induced pluripotent stem cells (iPSCs) and an extracellular matrix (ECM)-based hydrogel in a manner that is fully biocompatible. The reinforcement occurs as a post-fabrication step, which allows for the use of 3D-printing technology to generate thick, fully cellularized, and vascularized cardiac tissues. After tissue assembly and during the maturation process in a soft hydrogel, a small, tissue-penetrating reinforcer is deployed, leading to a significant increase in the tissue's mechanical properties. The tissue's robustness is demonstrated by injecting the tissue in a simulated minimally invasive procedure and showing that the tissue is functional and undamaged at the nano-, micro-, and macroscales.

Original languageEnglish
Article number2302229
JournalAdvanced Materials
Issue number31
StatePublished - 3 Aug 2023


  • 3D printing
  • ECM-based hydrogels
  • biomaterials
  • cardiac tissue engineering

All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Mechanical Engineering
  • General Materials Science


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