Population shift underlies Ca2+-induced regulatory transitions in the sodium-calcium exchanger (NCX)

Moshe Giladi; Reuben Hiller; Joel A. Hirsch; Daniel Khananshvili

doi:10.1074/jbc.M113.471698

Population shift underlies Ca²⁺-induced regulatory transitions in the sodium-calcium exchanger (NCX)

Moshe Giladi, Reuben Hiller, Joel A. Hirsch, Daniel Khananshvili

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Ca²⁺ binding to the regulatory two-domain tandem (CBD12) activates ion transport in NCX. Results: Ca²⁺ binding to high affinity sites of CBD12 results in a population shift, where more constraint conformational states become highly populated. Conclusion: Ca ²⁺-induced population shift governs NCX activation with no significant contribution of global conformational changes in CBD alignment. Significance: Population shift may represent a general mechanism for regulating NCX.

Original language	English
Pages (from-to)	23141-23149
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	32
DOIs	https://doi.org/10.1074/jbc.M113.471698
State	Published - 9 Aug 2013

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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title = "Population shift underlies Ca2+-induced regulatory transitions in the sodium-calcium exchanger (NCX)",

abstract = "Background: Ca2+ binding to the regulatory two-domain tandem (CBD12) activates ion transport in NCX. Results: Ca2+ binding to high affinity sites of CBD12 results in a population shift, where more constraint conformational states become highly populated. Conclusion: Ca 2+-induced population shift governs NCX activation with no significant contribution of global conformational changes in CBD alignment. Significance: Population shift may represent a general mechanism for regulating NCX.",

author = "Moshe Giladi and Reuben Hiller and Hirsch, \{Joel A.\} and Daniel Khananshvili",

year = "2013",

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AU - Giladi, Moshe

AU - Hiller, Reuben

AU - Hirsch, Joel A.

AU - Khananshvili, Daniel

PY - 2013/8/9

Y1 - 2013/8/9

N2 - Background: Ca2+ binding to the regulatory two-domain tandem (CBD12) activates ion transport in NCX. Results: Ca2+ binding to high affinity sites of CBD12 results in a population shift, where more constraint conformational states become highly populated. Conclusion: Ca 2+-induced population shift governs NCX activation with no significant contribution of global conformational changes in CBD alignment. Significance: Population shift may represent a general mechanism for regulating NCX.

AB - Background: Ca2+ binding to the regulatory two-domain tandem (CBD12) activates ion transport in NCX. Results: Ca2+ binding to high affinity sites of CBD12 results in a population shift, where more constraint conformational states become highly populated. Conclusion: Ca 2+-induced population shift governs NCX activation with no significant contribution of global conformational changes in CBD alignment. Significance: Population shift may represent a general mechanism for regulating NCX.

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DO - 10.1074/jbc.M113.471698

M3 - مقالة

C2 - 23798674

SN - 0021-9258

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

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ER -

Population shift underlies Ca²⁺-induced regulatory transitions in the sodium-calcium exchanger (NCX)

Abstract

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