Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy

Noam Zoref; Nadav Opatovski; Elias Nehme; Maytal Avrashami; Ilana Barzilai; Reut Kedem Orange; Boris Ferdman; Onit Alalouf; Dana Meron Azagury; Yosi Shamay; Yoav Shechtman

doi:10.1364/COSI.2023.JM2B.5

Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy

Noam Zoref, Nadav Opatovski, Elias Nehme, Maytal Avrashami, Ilana Barzilai, Reut Kedem Orange, Boris Ferdman, Onit Alalouf, Dana Meron Azagury, Yosi Shamay, Yoav Shechtman

Technion - Israel Institute of Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

We combine point spread function engineering with high-throughput microscopy for three-dimensional (3D) imaging. This includes novel hardware configurations and corresponding likelihood-based and neural-net-based image analysis, which is demonstrated on volumetric characterization of cancer cell spheroids.

Original language	American English
DOIs	https://doi.org/10.1364/COSI.2023.JM2B.5
State	Published - 1 Jan 2023
Event	2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023 - Boston, United States Duration: 14 Aug 2023 → 17 Aug 2023

Conference

Conference	2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023
Country/Territory	United States
City	Boston
Period	14/08/23 → 17/08/23

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Computer Science
Instrumentation
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering
Control and Systems Engineering
Space and Planetary Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1364/COSI.2023.JM2B.5

Cite this

Zoref, N., Opatovski, N., Nehme, E., Avrashami, M., Barzilai, I., Orange, R. K., Ferdman, B., Alalouf, O., Azagury, D. M., Shamay, Y., & Shechtman, Y. (2023). Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy. Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States. https://doi.org/10.1364/COSI.2023.JM2B.5

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title = "Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy",

abstract = "We combine point spread function engineering with high-throughput microscopy for three-dimensional (3D) imaging. This includes novel hardware configurations and corresponding likelihood-based and neural-net-based image analysis, which is demonstrated on volumetric characterization of cancer cell spheroids.",

author = "Noam Zoref and Nadav Opatovski and Elias Nehme and Maytal Avrashami and Ilana Barzilai and Orange, {Reut Kedem} and Boris Ferdman and Onit Alalouf and Azagury, {Dana Meron} and Yosi Shamay and Yoav Shechtman",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s).; 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023 ; Conference date: 14-08-2023 Through 17-08-2023",

year = "2023",

month = jan,

day = "1",

doi = "10.1364/COSI.2023.JM2B.5",

language = "American English",

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Zoref, N, Opatovski, N, Nehme, E, Avrashami, M, Barzilai, I, Orange, RK, Ferdman, B, Alalouf, O, Azagury, DM, Shamay, Y & Shechtman, Y 2023, 'Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy', Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States, 14/08/23 - 17/08/23. https://doi.org/10.1364/COSI.2023.JM2B.5

Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy. / Zoref, Noam; Opatovski, Nadav; Nehme, Elias et al.
2023. Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy

AU - Zoref, Noam

AU - Opatovski, Nadav

AU - Nehme, Elias

AU - Avrashami, Maytal

AU - Barzilai, Ilana

AU - Orange, Reut Kedem

AU - Ferdman, Boris

AU - Alalouf, Onit

AU - Azagury, Dana Meron

AU - Shamay, Yosi

AU - Shechtman, Yoav

PY - 2023/1/1

Y1 - 2023/1/1

N2 - We combine point spread function engineering with high-throughput microscopy for three-dimensional (3D) imaging. This includes novel hardware configurations and corresponding likelihood-based and neural-net-based image analysis, which is demonstrated on volumetric characterization of cancer cell spheroids.

AB - We combine point spread function engineering with high-throughput microscopy for three-dimensional (3D) imaging. This includes novel hardware configurations and corresponding likelihood-based and neural-net-based image analysis, which is demonstrated on volumetric characterization of cancer cell spheroids.

UR - http://www.scopus.com/inward/record.url?scp=85192537924&partnerID=8YFLogxK

U2 - 10.1364/COSI.2023.JM2B.5

DO - 10.1364/COSI.2023.JM2B.5

M3 - Paper

T2 - 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023

Y2 - 14 August 2023 through 17 August 2023

ER -

Point Spread Function Engineering for 3D Imaging in High-Throughput Microscopy

Abstract

Conference

All Science Journal Classification (ASJC) codes

UN SDGs

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