Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Asaf Grupi; Zehavit Shapira; Shimon Yudovich; Nurit Degani-Katzav; Shimon Weiss

doi:10.1016/j.bpr.2021.100007

Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Asaf Grupi, Zehavit Shapira, Shimon Yudovich, Nurit Degani-Katzav, Shimon Weiss

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA_A antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

Original language	English
Article number	100007
Journal	Biophysical Reports
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.bpr.2021.100007
State	Published - 8 Sep 2021

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology (miscellaneous)
Biophysics
Biochemistry
Biotechnology

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10.1016/j.bpr.2021.100007

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title = "Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs",

abstract = "Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (F{\"o}rster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.",

author = "Asaf Grupi and Zehavit Shapira and Shimon Yudovich and Nurit Degani-Katzav and Shimon Weiss",

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AU - Shapira, Zehavit

AU - Yudovich, Shimon

AU - Degani-Katzav, Nurit

AU - Weiss, Shimon

PY - 2021/9/8

Y1 - 2021/9/8

N2 - Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

AB - Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

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JO - Biophysical Reports

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ER -

Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Abstract

All Science Journal Classification (ASJC) codes

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