Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Asaf Grupi, Zehavit Shapira, Shimon Yudovich, Nurit Degani-Katzav, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

Abstract

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

Original languageEnglish
Article number100007
JournalBiophysical Reports
Volume1
Issue number1
DOIs
StatePublished - 8 Sep 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biophysics
  • Biochemistry
  • Biotechnology

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