Abstract
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.
Original language | English |
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Article number | 100007 |
Journal | Biophysical Reports |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - 8 Sep 2021 |
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Biophysics
- Biochemistry
- Biotechnology