@article{8ec1001dcb4f4544961cddcfb0bb2e73,
title = "Plum, an immunoglobulin superfamily protein, regulates axon pruning by facilitating TGF-β signaling",
abstract = "Axon pruning during development is essential for proper wiring of the mature nervous system, but itsregulation remains poorly understood. We have identified an immunoglobulin superfamily (IgSF) transmembrane protein, Plum, that is cell autonomously required for axon pruning of mushroom body (MB) γ neurons and for ectopic synapse refinement at the developing neuromuscular junction in. Drosophila. Plum promotes MB γ neuron axon pruning by regulating the expression of Ecdysone Receptor-B1, a key initiator of axon pruning. Genetic analyses indicate that Plum acts to facilitate signaling of Myoglianin, a glial-derived TGF-β, on MB γ neurons upstream of the type-I TGF-β receptor Baboon. Myoglianin, Baboon, and Ecdysone Receptor-B1 are also required for neuromuscular junction ectopic synapse refinement. Our study highlights both IgSF proteins and TGF-β facilitation as key promoters of developmental axon elimination and demonstrates a mechanistic conservation between MB axon pruning during metamorphosis and the refinement of ectopic larval neuromuscular connections",
author = "Yu, {Xiaomeng M.} and Itai Gutman and Mosca, {Timothy J.} and Engin Oezkan and Garcia, {K. Christopher} and Liqun Luo and Oren Schuldiner",
note = "National Institute of Child Health and Human Development; National Institutes of Health (NIH) [R37-NSO41044]; Israel Science Foundation [1864/08, 683/11]; Irving B. Harrison foundation; Estate of Florence and Charles Cuevas; Adelis Foundation; Epilepsy, Neonatology, and Developmental Biology Training grants (NIH [5132 NS 007280, HD007249]; Damon Runyon Cancer Research Foundation [DRG1993-08]We thank L. Attisano, T. Lee, M. B. O'Connor, N. Reist, the Vienna Drosophila RNAI Center, and the Bloomington stock center for reagents; the Fasll (1D4) and Brp (nc82) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa. We also thank R. Watts and E. Hoopfer for many hours of joint screening, N. Goriatcheva, D. Luginbuhl, N. Issman, and O. Fuchs for technical assistance, O. Golani for help with MATLAB programming, M. Hortsch and M. Serpe for discussions, and K. Shen, A. Yaron, M. Schuldiner, and members of the Luo and Schuldiner labs for discussions and their critical reading of our manuscript. This work was supported by a grant from the National Institutes of Health (NIH) (R37-NSO41044) (to L.L.), by grants from the Israel Science Foundation (1864/08 and 683/11) (Bio-med Legacy program), funds from the Irving B. Harrison foundation, the Estate of Florence and Charles Cuevas, and the Adelis Foundation (to O.S.). L.L. and K.C.G. are investigators of the Howard Hughes Medical Institute. O.S. is the incumbent of the Rothstein Career Development Chair of Genetic Diseases. T.J.M. was supported by Epilepsy, Neonatology, and Developmental Biology Training grants (NIH) (5132 NS 007280 and HD007249). X.M.Y. was a Damon Runyon Fellow supported by the Damon Runyon Cancer Research Foundation (DRG1993-08). We thank L. Attisano, T. Lee, M. B. O'Connor, N. Reist, the Vienna Drosophila RNAI Center, and the Bloomington stock center for reagents; the Fasll (1D4) and Brp (nc82) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa. We also thank R. Watts and E. Hoopfer for many hours of joint screening, N. Goriatcheva, D. Luginbuhl, N. Issman, and O. Fuchs for technical assistance, O. Golani for help with MATLAB programming, M. Hortsch and M. Serpe for discussions, and K. Shen, A. Yaron, M. Schuldiner, and members of the Luo and Schuldiner labs for discussions and their critical reading of our manuscript. This work was supported by a grant from the National Institutes of Health (NIH) (R37-NSO41044) (to L.L.), by grants from the Israel Science Foundation (1864/08 and 683/11) (Bio-med Legacy program), funds from the Irving B. Harrison foundation, the Estate of Florence and Charles Cuevas, and the Adelis Foundation (to O.S.). L.L. and K.C.G. are investigators of the Howard Hughes Medical Institute. O.S. is the incumbent of the Rothstein Career Development Chair of Genetic Diseases. T.J.M. was supported by Epilepsy, Neonatology, and Developmental Biology Training grants (NIH) (5132 NS 007280 and HD007249). X.M.Y. was a Damon Runyon Fellow supported by the Damon Runyon Cancer Research Foundation (DRG1993-08).",
year = "2013",
month = may,
day = "8",
doi = "10.1016/j.neuron.2013.03.004",
language = "الإنجليزيّة",
volume = "78",
pages = "456--468",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}