TY - JOUR
T1 - Physiological and Molecular Dissection of Daily Variance in Exercise Capacity
AU - Ezagouri, Saar
AU - Zwighaft, Ziv
AU - Sobel, Jonathan
AU - Baillieul, Sebastien
AU - Doutreleau, Stephane
AU - Ladeuix, Benjamin
AU - Golik, Marina
AU - Verges, Samuel
AU - Asher, Gad
N1 - We are grateful to the members of the Asher lab for their valuable comments on the manuscript. We thank Beni Sioani for his assistance with the animal care and Asher Auerbach for his technical support. G.A. is supported by the European Research Council (ERC-2017 CIRCOMMUNICATION 770869) and is a recipient of the EMBO young investigator award. The work done in the S.V. lab is supported by the “Fonds de dotation AGIR pour les maladies chroniques” and by the French National Research Agency (ANR-12-TECS-0010) in the framework of the “Investissements d’avenir” program (ANR-15-IDEX-02). J.S. is a recipient of a fellowship from the Placide Nicod Foundation.
PY - 2019/7/2
Y1 - 2019/7/2
N2 - Physical performance relies on the concerted action of myriad responses, many of which are under circadian clock control. Little is known, however, regarding the time-dependent effect on exercise performance at the molecular level. We found that both mice and humans exhibit daytime variance in exercise capacity between the early and late part of their active phase. The daytime variance in mice was dependent on exercise intensity and relied on the circadian clock proteins PER1/2. High-throughput gene expression and metabolic profiling of skeletal muscle revealed metabolic pathways that are differently activated upon exercise in a daytime-dependent manner. Remarkably, we discovered that ZMP, an endogenous AMPK activator, is induced by exercise in a time-dependent manner to regulate key steps in glycolytic and fatty acid oxidation pathways and potentially enhance exercise capacity. Overall, we propose that time of day is a major modifier of exercise capacity and associated metabolic pathways.
AB - Physical performance relies on the concerted action of myriad responses, many of which are under circadian clock control. Little is known, however, regarding the time-dependent effect on exercise performance at the molecular level. We found that both mice and humans exhibit daytime variance in exercise capacity between the early and late part of their active phase. The daytime variance in mice was dependent on exercise intensity and relied on the circadian clock proteins PER1/2. High-throughput gene expression and metabolic profiling of skeletal muscle revealed metabolic pathways that are differently activated upon exercise in a daytime-dependent manner. Remarkably, we discovered that ZMP, an endogenous AMPK activator, is induced by exercise in a time-dependent manner to regulate key steps in glycolytic and fatty acid oxidation pathways and potentially enhance exercise capacity. Overall, we propose that time of day is a major modifier of exercise capacity and associated metabolic pathways.
UR - http://www.scopus.com/inward/record.url?scp=85067884232&partnerID=8YFLogxK
U2 - 10.1016/j.cmet.2019.03.012
DO - 10.1016/j.cmet.2019.03.012
M3 - مقالة
SN - 1550-4131
VL - 30
SP - 78-91.e4
JO - Cell Metabolism
JF - Cell Metabolism
IS - 1
ER -