Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Nir A. Turko; Michael Isbach; Steffi Ketelhut; Burkhard Greve; Jürgen Schnekenburger; Natan T. Shaked; Björn Kemper

doi:10.1117/12.2252873

Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Nir A. Turko
, Michael Isbach
, Steffi Ketelhut
, Burkhard Greve
, Jürgen Schnekenburger
, Natan T. Shaked
, Björn Kemper

School of Biomedical Engineering

Tel Aviv University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

Original language	English
Title of host publication	Quantitative Phase Imaging III
Editors	Gabriel Popescu, YongKeun Park
Publisher	SPIE
ISBN (Electronic)	9781510605893
DOIs	https://doi.org/10.1117/12.2252873
State	Published - 1 Jan 2017
Event	Quantitative Phase Imaging III - San Francisco, United States Duration: 29 Jan 2017 → 31 Jan 2017

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10074

Conference

Conference	Quantitative Phase Imaging III
Country/Territory	United States
City	San Francisco
Period	29/01/17 → 31/01/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Digital holographic microscopy
Live cell imaging
Molecular specificity
Nanoparticles
Photothermal imaging
Quantitative phase imaging
Tumor cell biology

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2252873

Cite this

Turko, N. A., Isbach, M., Ketelhut, S., Greve, B., Schnekenburger, J., Shaked, N. T., & Kemper, B. (2017). Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup. In G. Popescu, & Y. Park (Eds.), Quantitative Phase Imaging III Article 100740Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10074). SPIE. https://doi.org/10.1117/12.2252873

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title = "Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup",

abstract = "We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.",

keywords = "Digital holographic microscopy, Live cell imaging, Molecular specificity, Nanoparticles, Photothermal imaging, Quantitative phase imaging, Tumor cell biology",

author = "Turko, \{Nir A.\} and Michael Isbach and Steffi Ketelhut and Burkhard Greve and J{\"u}rgen Schnekenburger and Shaked, \{Natan T.\} and Bj{\"o}rn Kemper",

note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Quantitative Phase Imaging III ; Conference date: 29-01-2017 Through 31-01-2017",

year = "2017",

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doi = "10.1117/12.2252873",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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booktitle = "Quantitative Phase Imaging III",

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Turko, NA, Isbach, M, Ketelhut, S, Greve, B, Schnekenburger, J, Shaked, NT & Kemper, B 2017, Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup. in G Popescu & Y Park (eds), Quantitative Phase Imaging III., 100740Q, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10074, SPIE, Quantitative Phase Imaging III, San Francisco, United States, 29/01/17. https://doi.org/10.1117/12.2252873

Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup. / Turko, Nir A.; Isbach, Michael; Ketelhut, Steffi et al.
Quantitative Phase Imaging III. ed. / Gabriel Popescu; YongKeun Park. SPIE, 2017. 100740Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10074).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

AU - Turko, Nir A.

AU - Isbach, Michael

AU - Ketelhut, Steffi

AU - Greve, Burkhard

AU - Schnekenburger, Jürgen

AU - Shaked, Natan T.

AU - Kemper, Björn

PY - 2017/1/1

Y1 - 2017/1/1

N2 - We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

AB - We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

KW - Digital holographic microscopy

KW - Live cell imaging

KW - Molecular specificity

KW - Nanoparticles

KW - Photothermal imaging

KW - Quantitative phase imaging

KW - Tumor cell biology

UR - https://www.scopus.com/pages/publications/85020440772

U2 - 10.1117/12.2252873

DO - 10.1117/12.2252873

M3 - Conference contribution

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Quantitative Phase Imaging III

A2 - Popescu, Gabriel

A2 - Park, YongKeun

PB - SPIE

T2 - Quantitative Phase Imaging III

Y2 - 29 January 2017 through 31 January 2017

ER -

Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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