Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Nir A. Turko, Michael Isbach, Steffi Ketelhut, Burkhard Greve, Jürgen Schnekenburger, Natan T. Shaked, Björn Kemper

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

Original languageEnglish
Title of host publicationQuantitative Phase Imaging III
EditorsGabriel Popescu, YongKeun Park
PublisherSPIE
ISBN (Electronic)9781510605893
DOIs
StatePublished - 1 Jan 2017
EventQuantitative Phase Imaging III - San Francisco, United States
Duration: 29 Jan 201731 Jan 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10074

Conference

ConferenceQuantitative Phase Imaging III
Country/TerritoryUnited States
CitySan Francisco
Period29/01/1731/01/17

Keywords

  • Digital holographic microscopy
  • Live cell imaging
  • Molecular specificity
  • Nanoparticles
  • Photothermal imaging
  • Quantitative phase imaging
  • Tumor cell biology

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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