Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems

D. Aerssens; L. Miret-Casals; D. Gomez; D. Sousa-Neves; Y. Levy; M. De Vleesschouwer; A. Manicardi; A. Madder

doi:https://doi.org/10.1039/d2ob02181a

Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems

D. Aerssens, L. Miret-Casals, D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, A. Manicardi, A. Madder

Research output: Contribution to journal › Article › peer-review

Abstract

We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)₃ sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.

Original language	English
Pages (from-to)	5648-5657
Number of pages	10
Journal	Organic and Biomolecular Chemistry
Volume	21
Issue number	27
DOIs	https://doi.org/10.1039/d2ob02181a
State	Published - 21 Jun 2023
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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Aerssens, D., Miret-Casals, L., Gomez, D., Sousa-Neves, D., Levy, Y., De Vleesschouwer, M., Manicardi, A., & Madder, A. (2023). Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems. Organic and Biomolecular Chemistry, 21(27), 5648-5657. https://doi.org/10.1039/d2ob02181a

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title = "Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems",

abstract = "We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)3 sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.",

author = "D. Aerssens and L. Miret-Casals and D. Gomez and D. Sousa-Neves and Y. Levy and {De Vleesschouwer}, M. and A. Manicardi and A. Madder",

note = "Publisher Copyright: {\textcopyright} 2023 The Royal Society of Chemistry.",

year = "2023",

month = jun,

day = "21",

doi = "https://doi.org/10.1039/d2ob02181a",

language = "الإنجليزيّة",

volume = "21",

pages = "5648--5657",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

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number = "27",

}

Aerssens, D, Miret-Casals, L, Gomez, D, Sousa-Neves, D, Levy, Y, De Vleesschouwer, M, Manicardi, A & Madder, A 2023, 'Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems', Organic and Biomolecular Chemistry, vol. 21, no. 27, pp. 5648-5657. https://doi.org/10.1039/d2ob02181a

TY - JOUR

T1 - Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems

AU - Aerssens, D.

AU - Miret-Casals, L.

AU - Gomez, D.

AU - Sousa-Neves, D.

AU - Levy, Y.

AU - De Vleesschouwer, M.

AU - Manicardi, A.

AU - Madder, A.

PY - 2023/6/21

Y1 - 2023/6/21

N2 - We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)3 sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.

AB - We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)3 sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.

UR - http://www.scopus.com/inward/record.url?scp=85164299411&partnerID=8YFLogxK

U2 - https://doi.org/10.1039/d2ob02181a

DO - https://doi.org/10.1039/d2ob02181a

M3 - مقالة

C2 - 37394986

SN - 1477-0520

VL - 21

SP - 5648

EP - 5657

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 27

ER -

Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems

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