Peptide interaction with and insertion into membranes

Ron Saar-Dover, Avraham Ashkenazi, Yechiel Shai

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Natural and synthetic membrane active peptides as well as fragments from membrane proteins interact with membranes. In several cases, such interactions cause the insertion of the peptides to the membrane and their assembly within the lipid bilayer. Here we present spectroscopic approaches utilizing NBD and rhodamine fluorescently labeled peptides to measure peptide-membrane interaction and peptide-peptide interaction within the membrane. The usage of the physical properties of NBD and rhodamine in solution and in membranes provides useful information on the interplay between peptides and lipids.

Original languageAmerican English
Title of host publicationMembrane Biogenesis
Subtitle of host publicationMethods and Protocols
EditorsDoron Rapaport, Johannes Herrmann, D. Rapaport, J. Herrmann
PublisherHumana Press
Pages173-183
Number of pages11
ISBN (Electronic)978-1-62703-487-6
ISBN (Print)9781627034869
DOIs
StatePublished - 20 Sep 2013

Publication series

NameMethods in Molecular Biology
Volume1033

Keywords

  • ATR-FTIR
  • FRET
  • Fluorescence spectroscopy
  • Liposomes
  • NBD
  • Peptide-membrane interaction
  • Rhodamine

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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