@article{ae99f43259ed490d8a964d14f0a564fa,
title = "Paternal Mitochondrial Destruction after Fertilization Is Mediated by a Common Endocytic and Autophagic Pathway in Drosophila",
abstract = "Almost all animals contain mitochondria of maternal origin only, but the exact mechanisms underlying this phenomenon are still vague. We investigated the fate of Drosophila paternal mitochondria after fertilization. We demonstrate that the sperm mitochondrial derivative (MD) is rapidly eliminated in a stereotypical process dubbed paternal mitochondrial destruction (PMD). PMD is initiated by a network of vesicles resembling multivesicular bodies and displaying common features of the endocytic and autophagic pathways. These vesicles associate with the sperm tail and mediate the disintegration of its plasma membrane. Subsequently, the MD separates from the axoneme and breaks into smaller fragments, which are then sequestered by autophagosomes for degradation in lysosomes. We further provide evidence for the involvement of the ubiquitin pathway and the autophagy receptor p62 in this process. Finally, we show that the ubiquitin ligase Parkin is not involved in PMD, implying a divergence from the autophagic pathway of damaged mitochondria.",
author = "Yoav Politi and Liron Gal and Yossi Kalifa and Liat Ravid and Zvulun Elazar and Eli Arama",
note = "TRiP at Harvard Medical School (NIH/National Institute of General Medical Sciences) [R01-GM084947]; European Research Council under the European Union [616088]; Minerva Foundation; German Federal Ministry of Education and Research; Israel Science Foundation [308/09, 921/13, 535/11]; Ministry of Agriculture of the State of Israel; Israel Cancer Research Fund; Yeda-Sela Center for Basic Research; Y. Leon Benoziyo Institute for Molecular Medicine; German-Israeli Foundation [1129-157]We are grateful to Uri Abdu, Hugo Bellen, Marie-Helene Bre, Shari Carmon, Aaron DiAntonio, Sebastien Gaumer, Thomas P. Neufeld, Ioannis P. Nezis, Leo J. Pallanck, Tor-Erik Rusten, Ben-Zion Shilo, Avraham Yaron, and the Bloomington Stock Center for providing additional stocks and reagents. We thank the TRiP at Harvard Medical School (NIH/National Institute of General Medical Sciences grant R01-GM084947) for providing the transgenic RNAi fly stocks used in this study. We also thank the E. A. laboratory members for encouragement and advice. We are indebted to Vera Shinder, Smadar Zaidman, and Eyal Shimoni for help and guidance with the EM studies, Ofra Golani for MATLAB data analysis and quantification, and Bat-Chen Tamin-Yecheskel and Hanoch Tempelhof, who helped to carry out experiments during their rotation periods in the laboratory. We warmly thank Samara Brown for editing the manuscript. This research was supported in part by grants from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC grant agreement (616088), the Minerva Foundation with funding from the German Federal Ministry of Education and Research, the Israel Science Foundation (308/09 and 921/13), the Ministry of Agriculture of the State of Israel, the Israel Cancer Research Fund, and the Yeda-Sela Center for Basic Research. E. A. is also supported by a grant from the Y. Leon Benoziyo Institute for Molecular Medicine. E. A. is the Incumbent of the Corinne S. Koshland Car",
year = "2014",
month = may,
doi = "https://doi.org/10.1016/j.devcel.2014.04.005",
language = "الإنجليزيّة",
volume = "29",
pages = "305--320",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",
}