Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting

Roy Kelner; Joseph Rosen

doi:https://doi.org/10.1364/OE.24.002200

Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting

Roy Kelner, Joseph Rosen

Department of Electrical & Computer Engineering

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.

Original language	American English
Pages (from-to)	2200-2214
Number of pages	15
Journal	Optics Express
Volume	24
Issue number	3
DOIs	https://doi.org/10.1364/OE.24.002200
State	Published - 8 Feb 2016

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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Cite this

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title = "Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting",

abstract = "The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.",

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AB - The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.

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Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting

Abstract

All Science Journal Classification (ASJC) codes

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