PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers

Michal Moyal Ben Zvi, Elena Shklarman, Tania Masci, Haim Kalev, Thomas Debener, Sharoni Shafir, Marianna Ovadis, Alexander Vainstein

Research output: Contribution to journalArticlepeer-review

Abstract

• Floral scent is a complex trait of biological and applied significance. To evaluate whether scent production originating from diverse metabolic pathways (e.g. phenylpropanoids and isoprenoids) can be affected by transcriptional regulators, Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1) transcription factor was introduced into Rosa hybrida. • Color and scent profiles of PAP1-transgenic and control (β-glucuronidase-expressing) rose flowers and the expression of key genes involved in the production of secondary metabolites were analyzed. To evaluate the significance of the scent modification, olfactory trials were conducted with both humans and honeybees. • In addition to increased levels of phenylpropanoid-derived color and scent compounds when compared with control flowers, PAP1-transgenic rose lines also emitted up to 6.5 times higher levels of terpenoid scent compounds. Olfactory assay revealed that bees and humans could discriminate between the floral scents of PAP1-transgenic and control flowers. • The increase in volatile production in PAP1 transgenes was not caused solely by transcriptional activation of their respective biosynthetic genes, but probably also resulted from enhanced metabolic flux in both the phenylpropanoid and isoprenoid pathways. The mechanism(s) governing the interactions in these metabolic pathways that are responsible for the production of specialized metabolites remains to be elucidated.

Original languageAmerican English
Pages (from-to)335-345
Number of pages11
JournalNEW PHYTOLOGIST
Volume195
Issue number2
DOIs
StatePublished - Jul 2012

Keywords

  • Olfactory
  • PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1)
  • Phenylpropanoid
  • Rosa hybrida
  • Scent
  • Terpenoid

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Fingerprint

Dive into the research topics of 'PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers'. Together they form a unique fingerprint.

Cite this