Packaging of DNA origami in viral capsids

Idit Kopatz; Ran Zalk; Yael Levi-Kalisman; Efrat Zlotkin-Rivkin; Gabriel A. Frank; Stanislav Kler

doi:10.1039/c8nr10113b

Packaging of DNA origami in viral capsids

Idit Kopatz, Ran Zalk, Yael Levi-Kalisman, Efrat Zlotkin-Rivkin, Gabriel A. Frank, Stanislav Kler

Ben-Gurion University of the Negev, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

Original language	American English
Pages (from-to)	10160-10166
Number of pages	7
Journal	Nanoscale
Volume	11
Issue number	21
DOIs	https://doi.org/10.1039/c8nr10113b
State	Published - 7 Jun 2019

All Science Journal Classification (ASJC) codes

General Materials Science

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10.1039/c8nr10113b

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title = "Packaging of DNA origami in viral capsids",

abstract = "Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.",

author = "Idit Kopatz and Ran Zalk and Yael Levi-Kalisman and Efrat Zlotkin-Rivkin and Frank, \{Gabriel A.\} and Stanislav Kler",

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AU - Kopatz, Idit

AU - Zalk, Ran

AU - Levi-Kalisman, Yael

AU - Zlotkin-Rivkin, Efrat

AU - Frank, Gabriel A.

AU - Kler, Stanislav

PY - 2019/6/7

Y1 - 2019/6/7

N2 - Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

AB - Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

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U2 - 10.1039/c8nr10113b

DO - 10.1039/c8nr10113b

M3 - Article

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SP - 10160

EP - 10166

JO - Nanoscale

JF - Nanoscale

IS - 21

ER -

Packaging of DNA origami in viral capsids

Abstract

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