Oxygen insensitive amperometric glucose biosensor based on FAD dependent glucose dehydrogenase co-entrapped with DCPIP or DCNQ in a polydopamine layer

Roy Cohen, Yifat Cohen, Dina Mukha, Omer Yehezkeli

Research output: Contribution to journalArticlepeer-review

Abstract

Continuous glucose monitoring devices (CGM) are the present and the future for the challenging task of diabetes management. With a yearly increase of patients worldwide, glucose biosensors with high accuracy and low interference effect become crucial and should be developed. Here we show the development of an oxygen insensitive glucose biosensor which comprises FAD-dependent glucose dehydrogenase, dichlorophenol indophenol (DCPIP) or 2,3-dichloro-naphthoquinone (DCNQ) as redox mediators and polydopamine as a constraining layer. The sensors were fully characterized and the effect of interferences on glucose-sensing was analyzed. We further show long term bioelectrocatalytic activity for at least 3 days without any additional coating or treatment. The system shows great reproducibility via an easy fabrication methodology that should allow low-cost manufacturing. Those encouraging results show great promise for future sensor development of enzyme-based bioelectronics devices.

Original languageEnglish
Article number137477
JournalElectrochimica Acta
Volume367
DOIs
StatePublished - 20 Jan 2021

Keywords

  • FAD-GDH
  • Glucose sensing
  • MWCNTs
  • Nano-bio interface
  • Polydopamine
  • Redox mediator

All Science Journal Classification (ASJC) codes

  • General Chemical Engineering
  • Electrochemistry

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