Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics

Ilia Kaminker, Ryan Barnes, Songi Han

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (< 1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Number of pages27
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Enzymology


  • Hydration dynamics
  • Overhauser dynamic nuclear polarization
  • Protein hydration water measurements
  • Site-directed hydration dynamics
  • Site-directed spin labeling

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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