Osteoclast Methods in Protein Phosphatase Research

Nina Reuven, Maayan Barnea-Zohar, Ari Elson

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Osteoclasts are specialized cells that degrade bone and are essential for bone formation and maintaining bone homeostasis. Excess or deficient activity of these cells can significantly alter bone mass, structure, and physical strength, leading to significant morbidity, as in osteoporosis or osteopetrosis, among many other diseases. Protein phosphorylation in osteoclasts plays critical roles in the signaling pathways that govern the production of osteoclasts and regulate their bone-resorbing activity. In this chapter, we describe the isolation of mouse splenocytes and their differentiation into mature osteoclasts on resorptive (e.g., bone) and non-resorptive (e.g., plastic or glass) surfaces, examining matrix resorption by osteoclasts, immunofluorescence staining of these cells, and knocking out genes by CRISPR in the mouse osteoclastogenic cell line RAW264.7.

Original languageEnglish
Title of host publicationProtein Tyrosine Phosphatases
Subtitle of host publicationMethods and Protocols
EditorsDamien Thevenin, Jorg P. Muller
PublisherHumana Press
Pages57-79
Number of pages23
ISBN (Electronic)978-1-0716-3569-8
ISBN (Print)978-1-0716-3568-1
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2743
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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