@inbook{68d1721f7e284effb3165bfcfd083489,
title = "Osteoclast Methods in Protein Phosphatase Research",
abstract = "Osteoclasts are specialized cells that degrade bone and are essential for bone formation and maintaining bone homeostasis. Excess or deficient activity of these cells can significantly alter bone mass, structure, and physical strength, leading to significant morbidity, as in osteoporosis or osteopetrosis, among many other diseases. Protein phosphorylation in osteoclasts plays critical roles in the signaling pathways that govern the production of osteoclasts and regulate their bone-resorbing activity. In this chapter, we describe the isolation of mouse splenocytes and their differentiation into mature osteoclasts on resorptive (e.g., bone) and non-resorptive (e.g., plastic or glass) surfaces, examining matrix resorption by osteoclasts, immunofluorescence staining of these cells, and knocking out genes by CRISPR in the mouse osteoclastogenic cell line RAW264.7.",
author = "Nina Reuven and Maayan Barnea-Zohar and Ari Elson",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "https://doi.org/10.1007/978-1-0716-3569-8_4",
language = "الإنجليزيّة",
isbn = "978-1-0716-3568-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "57--79",
editor = "Damien Thevenin and Muller, {Jorg P.}",
booktitle = "Protein Tyrosine Phosphatases",
address = "الولايات المتّحدة",
}