Osmotic erythrocyte lysis for chemical- and label-free impedance cytometry

T. E. Winkler, H. Ben-Yoav, D. L. Kelly, R. Ghodssi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present the first implementation of osmotic erythrocyte (RBC) lysis for microfluidic leukocyte (WBC) impedance cytometry. The method relies on osmotic pressure differentials to rupture RBC membranes, and detection of WBCs based on changes in impedance. Specifically, we demonstrate an up to 1,200-fold decrease in noise from RBCs in WBC recordings. Our approach enables lab-on-a-chip (LOC)-based WBC counting on whole blood samples without traditionally required chemicals or labels.

Original languageAmerican English
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Pages351-354
Number of pages4
ISBN (Electronic)9781479989553
DOIs
StatePublished - 5 Aug 2015
Externally publishedYes
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: 21 Jun 201525 Jun 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Conference

Conference18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/TerritoryUnited States
CityAnchorage
Period21/06/1525/06/15

Keywords

  • Microsystem integration
  • impedance cytometry
  • lab-on-a-chip
  • microfluidics
  • osmotic lysis

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

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