Optical modulation biosensing system for rapid detection of biological targets at low concentrations

Michael Margulis; Meir Cohen; Shmuel Burg; Shira Avivi-Mintz; Amos Danielli

doi:10.1364/BOE.430410

Optical modulation biosensing system for rapid detection of biological targets at low concentrations

Michael Margulis, Meir Cohen, Shmuel Burg, Shira Avivi-Mintz, Amos Danielli

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.

Original language	English
Pages (from-to)	5338-5350
Number of pages	13
Journal	Biomedical Optics Express
Volume	12
Issue number	9
DOIs	https://doi.org/10.1364/BOE.430410
State	Published - 1 Sep 2021

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.430410

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title = "Optical modulation biosensing system for rapid detection of biological targets at low concentrations",

abstract = "In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96\% sensitivity and 100\% specificity.",

author = "Michael Margulis and Meir Cohen and Shmuel Burg and Shira Avivi-Mintz and Amos Danielli",

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T1 - Optical modulation biosensing system for rapid detection of biological targets at low concentrations

AU - Margulis, Michael

AU - Cohen, Meir

AU - Burg, Shmuel

AU - Avivi-Mintz, Shira

AU - Danielli, Amos

PY - 2021/9/1

Y1 - 2021/9/1

N2 - In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.

AB - In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.

UR - http://www.scopus.com/inward/record.url?scp=85112861928&partnerID=8YFLogxK

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DO - 10.1364/BOE.430410

M3 - مقالة

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SN - 2156-7085

VL - 12

SP - 5338

EP - 5350

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 9

ER -

Optical modulation biosensing system for rapid detection of biological targets at low concentrations

Abstract

All Science Journal Classification (ASJC) codes

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