Optical detection of epigenetic marks: Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Yael Michaeli; Tamar Shahal; Dmitry Torchinsky; Assaf Grunwald; Rony Hoch; Yuval Ebenstein

doi:10.1039/c3cc42543f

Optical detection of epigenetic marks: Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Yael Michaeli, Tamar Shahal, Dmitry Torchinsky, Assaf Grunwald, Rony Hoch, Yuval Ebenstein

School of Chemistry

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Original language	English
Pages (from-to)	8599-8601
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	77
DOIs	https://doi.org/10.1039/c3cc42543f
State	Published - 29 Aug 2013

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Optical detection of epigenetic marks: Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases",

abstract = "Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.",

author = "Yael Michaeli and Tamar Shahal and Dmitry Torchinsky and Assaf Grunwald and Rony Hoch and Yuval Ebenstein",

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T1 - Optical detection of epigenetic marks

T2 - Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

AU - Michaeli, Yael

AU - Shahal, Tamar

AU - Torchinsky, Dmitry

AU - Grunwald, Assaf

AU - Hoch, Rony

AU - Ebenstein, Yuval

PY - 2013/8/29

Y1 - 2013/8/29

N2 - Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

AB - Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

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U2 - 10.1039/c3cc42543f

DO - 10.1039/c3cc42543f

M3 - مقالة

SN - 1359-7345

VL - 49

SP - 8599

EP - 8601

JO - Chemical Communications

JF - Chemical Communications

IS - 77

ER -

Optical detection of epigenetic marks: Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Abstract

All Science Journal Classification (ASJC) codes

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