On-line biosensor for the detection of putative toxicity in water contaminants

Potential threat on drinking water requires monitoring solutions, such as the one proposed herein, as a real-time, wide ranged, water monitoring system to detect the presence of toxicants in water. We studied the role of a selected number of parameters affecting performance and, thus, improved the prototype into an optimized next-generation device, resulting in enabling increased measurement duration, coupled with increased sensitivity. The chosen parameters in question were the peristaltic flow system, the fiber probe matrix stability through a re-design of the fiber probe holder and flow unit cell, as well as the modulation of bacterial medium concentration to increase bioreporter performance while keeping biofouling in check. Measurements were made with spiked samples and validated with polluted field-collected samples.