OffRisk: A docker image for annotating CRISPR off-target sites in the human genome

Gil Ad Barkai; Tal Malul; Yossi Eliaz; Eran Eyal; Isana Veksler-Lublinsky

doi:https://doi.org/10.1093/bioadv/vbad138

OffRisk: A docker image for annotating CRISPR off-target sites in the human genome

Gil Ad Barkai, Tal Malul, Yossi Eliaz, Eran Eyal, Isana Veksler-Lublinsky

Department of Software and Information Systems Engineering

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

The CRISPR-Cas9 system has been adapted to achieve targeted genome editing as well as transcriptional control by customizing 20-nt guide RNA (gRNA) molecules for desired regions in the target genome. Designing gRNAs must consider nonspecific and unintended binding, known as off-targets, since these may have potentially harmful effects. To assist in gRNA design, we have developed OffRisk. This Docker-based tool annotates off-target sites in the human genome and assigns them a potential risk label by incorporating functional and regulatory information at different molecular levels.

Original language	American English
Article number	vbad138
Journal	Bioinformatics Advances
Volume	3
Issue number	1
DOIs	https://doi.org/10.1093/bioadv/vbad138
State	Published - 1 Jan 2023

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology
Structural Biology
Computer Science Applications

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OffRisk: A docker image for annotating CRISPR off-target sites in the human genome

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