Observing DNA in live cells

Lucien E. Weiss; Tal Naor; Yoav Shechtman

doi:10.1042/BST20170301

Observing DNA in live cells

Lucien E. Weiss, Tal Naor, Yoav Shechtman

Biomedical Engineering

Technion - Israel Institute of Technology

Research output: Contribution to journal › Review article › peer-review

Abstract

The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.

Original language	English
Pages (from-to)	729-740
Number of pages	12
Journal	Biochemical Society Transactions
Volume	46
Issue number	3
DOIs	https://doi.org/10.1042/BST20170301
State	Published - 19 Jun 2018

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1042/BST20170301

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title = "Observing DNA in live cells",

abstract = "The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.",

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AU - Shechtman, Yoav

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N2 - The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.

AB - The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.

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U2 - 10.1042/BST20170301

DO - 10.1042/BST20170301

M3 - مقالة مرجعية

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JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

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ER -

Observing DNA in live cells

Abstract

All Science Journal Classification (ASJC) codes

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